Experiment+1-Microbes

=__**Experiment 1-Microbes**__=

__**FUNCTIONS OF THE PARTS OF THE MICROSCOPE**__
1. Eyepiece 2. Coarse Adjustment 3. Fine Adjustment 4. Objectives 5. Arm 6. Inclination Joint 7. Body Tube 8. Revolving Nose Piece 9. Objectives 10. Objectives 11. Stage Clips 12. Stage 13. Diaphragm 14. Light Source 15. Base 16. ABB condenser

__PICTURES TAKEN FROM THE MICROSCOPE__
Scale Bar Image 4x Magnification

Amphibian 4x Magnification

Amphibian 10x Magnification

Lily Anther 4x Magnification

Lily Anther 10x Magnification

Lily Anther Pollen 4x Magnification

Lily Anther Pollen 10x Magnification

1. Hook up the microscope to the computer. 2. Open the program Motic Images 2000. You will see the tool bar on the top of the screen. 3. On the tool bar choose:  Options --> Calibration ---> Calibration Wizard (a new window will open)  a. Change "objective magnification" to the respective lens you are using (4x in this case).  b. Change "External Diameter" to 600 um. 4. Click "Calibration" once. 5. Click "Capture Immediately" once. The image of the circle should appear. If not, make sure the microscope and camera are both "on." Also, make sure that the silver rod is pulled outward, this will stop it from blocking the picture. 6. Capture --> still image. This will open a new window (check behind the Motic DS capture window). 7. Choose: Options -> Calibration -> Calibration Table  As the calibration table appears check the following: a. Scale of 4x sign ="true". b. Sign image width = 640 pixels. c. Sign image height = 480 pixels. d. Calibration of 4x sign = "true" <span style="font-family: Arial,Helvetica;">e. Check the box 0 next to Scale in the lower left corner of the Calibration Table. <span style="font-family: Arial,Helvetica;">f. Click OK. <span style="font-family: Arial,Helvetica;">8. Now go to File --> save/or save as --> give a name to the file with .jpg extension --> Save. <span style="font-family: Arial,Helvetica;">Make sure you know where you are saving your image. You will need to retrieve this image later and email it to yourself.

**Testing the Calibration**
<span style="font-family: Arial,Helvetica; font-size: medium;">1. Use a slide with known measurements, a calibration circle and micrometer scale on it. <span style="font-family: Arial,Helvetica;">2. Click File -> "Capture window" on the tool bar.

<span style="font-family: Arial,Helvetica;">You can also acquire this step by choosing the video camera icon on the toolbar.

<span style="font-family: Arial,Helvetica;">Note: when still images are recorded with the video camera icon they will be 640 x 480 pixels. <span style="font-family: Arial,Helvetica;">3. Slide the silver lever outward on the microscope head until an image appears in the capture window.

<span style="font-family: Arial,Helvetica;">In the Motic DS Capture Window: Click Capture -> still image. This should open a window at the bottom of the Motic Images 2000 screen. <span style="font-family: Arial,Helvetica;">4. In the Motic Images 2000 window use toolbar as follows:

<span style="font-family: Arial,Helvetica;">Measure -> click on "straight line" icon. Come back to the Still image. <span style="font-family: Arial,Helvetica;">5. Pick two points on the circle (usually the edges) and draw a line between two points. <span style="font-family: Arial,Helvetica;">6. In the extreme left corner of the bottom window, the lens should correspond to the lens of the microscope that you are using to capture the image.

__<span style="font-family: Arial,Helvetica;">MICROORGANISMS AND THE DISEASES THEY MIGHT CAUSE __
Bacteria: <span style="background-color: #ffffff; color: #cd1313; font-family: arial,sans-serif; font-size: small;">A member of a large group of unicellular microorganisms  <span style="background-color: #ffffff; color: #cd1313; font-family: arial,sans-serif; font-size: small;"> lacking organelles and an organized nucleus, including  <span style="background-color: #ffffff; color: #cd1313; font-family: arial,sans-serif; font-size: small;">some that can cause disease. Bacteria on the human flora has the potential to cause pneumonia, meningitis, and many other different sicknesses and diseases.

Cocci:
<span style="background-color: #ffffff; color: #ff00ff; font-family: arial,sans-serif; font-size: small;">Any spherical or roughly spherical bacterium. Cocci may cause strep throat, impetigo, ear infections, and was the cause of scarlet fever.

Fungus:
<span style="background-color: #ffffff; color: #808080; font-family: arial,sans-serif; font-size: small;">Any of a group of unicellular, multicellular, or syncytial <span style="background-color: #ffffff; color: #808080; font-family: arial,sans-serif; font-size: small;"> spore-producing organisms feeding on organic matter, <span style="background-color: #ffffff; color: #808080; font-family: arial,sans-serif; font-size: small;">including molds, yeast, etc. If fungus enters the body there are numerous diseases and illnesses that may be caused. For example, high cholesterol, kidney stones, cancer, diabetes, and many more.

Protozoa:
<span style="background-color: #ffffff; color: #008000; font-family: arial,sans-serif; font-size: small;">A phylum or grouping of phyla that comprises the <span style="background-color: #ffffff; color: #008000; font-family: arial,sans-serif; font-size: small;">single-celled microscopic animals, which include <span style="background-color: #ffffff; color: #008000; font-family: arial,sans-serif; font-size: small;">amebas, flagellates, ciliates, etc. Protozoas may cause malaria, diarrhea, amoebic meningitis, toxoplasmosis, and many others.